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. 2009 Oct 19;284(50):34809–34818. doi: 10.1074/jbc.M109.039040

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — The effect of tRNA levels on stalling and tagging. A, one or more Pro codons in the WPPPSI sequence was switched to CCC (lowercase p) so that it would only be recognized by Pro2 tRNA. An uppercase P represents a Pro codon not recognized by Pro2 tRNA. Tagging of the GST-WPPPSI fusion was monitored in the presence or absence of a plasmid (pRARE) overexpressing the Pro2 tRNA. pRARE also expresses several other rare tRNAs. B, stalling sequences containing one or three rare Arg codons (AGG) were fused to the C terminus of GST. Tagging by tmRNA-H was monitored with anti-His₆ antibodies in the presence or absence of a plasmid (pRARE) overexpressing the cognate tRNA, Arg5.