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Efficiency of stalling of the SecM and FXXYXIWPPP peptides
The peptide sequences shown were inserted into the full-length lacZ gene following the ninth codon. β-Galactosidase activity is shown in Miller units along with the standard deviation. The Ala substitutions in bold are known to prevent stalling in SecM and FxxYxIWPPP.
| Stalling peptide | LacZ activity |
|---|---|
| FSTPVWISQAQGIRAGP | 8.2 ± 2.0 |
| FSTPVWISQAQGIRAGA | 10,812 ± 1,288 |
| SLQKRLFQKYGIWPPPSI | 117 ± 14 |
| SLQKRLFQKYGIWPAPSI | 11,172 ± 3,580 |
