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. 2009 Oct 23;284(50):34880–34888. doi: 10.1074/jbc.M109.068239

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Antagonism of UFA-dependent DNA binding by the presence of SFA ligands. A, competition of 16:0-CoA with 18:1Δ9-CoA. Lane 1, FabR only. Lanes 2–7, FabR DNA binding was activated by the presence of 1 μm 18:1Δ9-CoA and the completion experiment was performed with 16:0-CoA concentrations from 0 to 3.2 μm. B, competition between 18:1Δ11-ACP and 16:0-ACP. Lane 1, FabR alone. Lanes 2–7, FabR DNA binding was activated by 0.1 μm 18:1Δ11-ACP and antagonized with 16:0-ACP using concentrations between 0 and 0.32 μm.