TABLE 1.

FabR regulation of fatty acid composition

Strains were grown in Luria broth at 37 °C, harvested in the mid-log phase of growth, the lipids were extracted, fatty acid methyl esters were prepared, and the compositions were determined by gas-liquid chromatography as described under “Experimental Procedures.” wt, wild-type.

Strain	Relevant genotype	Plasmid	Source	Fatty acid composition
				16:0	18:0	16:1a	18:1a	UFA/SFA
UB1005	wtb		Ref. 37	48.1	2.6	28.2	21.0	0.97
KZ6c	fabR		This work	30.1	1.6	13.0	55.3	2.2
KZ7c	yijD		This work	48.0	1.5	32.2	18.1	1.0
UB1005	wt	pBlued	This work	50.7	4.6	31.1	13.3	0.80
UB1005	wt	pFabR	This work	52.1	2.1	29.2	16.5	0.84
UB1005	wt	pYijD	This work	50.5	2.4	28.9	18.0	0.88
UB1005	wt	pFabR-YijD	This work	55.8	1.4	27.9	14.7	0.75
KZ6	fabR	pFabR	This work	51.7	1.9	27.4	18.8	0.86
BW25113	Keioe		Ref. 17	32.7	2.3	24.1	40.8	1.9
JW3935f	fabR		Ref. 17	30.4	1.3	23.8	44.1	2.1
JW3936f	yijD		Ref. 17	32.5	2.2	23.3	41.8	1.9
BW25113	Keio	pFabR	This work	54.0	1.7	20.2	23.5	0.78

^a The contributions of the respective cyclopropane derivatives were included in these percentages.

^b Strain UB1005 (gyrA37, metB1, relA1, spoT1, LAM⁻, LAM^R, F⁻) (37).

^c Strains KZ6 and KZ7 were derivatives of strain UB1005.

^d The pBluescript II KS (Stratagene) plasmid was used as empty vector control, and all of the other expression plasmids were constructed in this vector.

^e Strain BW25113 (Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), LAM⁻, rph-1, hsdR514, Δ(rhaD-rhaB)568, F⁻) was the parent strain for the Keio collection.

^f Strains JW3935 and JW3936 were derivatives of strain BW25113.