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. 2009 Oct 11;284(50):34901–34910. doi: 10.1074/jbc.M109.038356

FIGURE 6.

FIGURE 6.

HDAC1 6Q represses the deacetylase activity of the HDAC1-containing protein complexes. A, HDAC1 can form homo- or heterodimers in the corepressor complex. The nuclear extracts from 3134 cells, which overexpressed FLAG-tagged HDAC1, were prepared and subsequently immunoprecipitated (IP) with FLAG and mSin3A antibodies. The presence of HDAC2 as well as endogenous and FLAG-tagged HDAC1 was examined by Western blot. B, HDAC1 containing protein complexes were purified from 3134 cells overexpressing FLAG-tagged HDAC1. HDAC1 complexes were then incubated with purified HDAC1 or HDAC1 6Q and acetylated histones to examine their overall deacetylase activity. The wild type HDAC1 from the complex and recombinant HDAC1 6Q levels were examined by Western blot (WB) with HDAC1 antibody. The presence of HDAC2, mSin3A, Mi2, or CoREST from the complexes were also examined. Lane 1, acetylated histone input; lane 2, HDAC1 6Q input; lane 3, HDAC1 input; lane 4, HDAC1 complex alone; lane 5, HDAC1 complex with HDAC1 6Q in the reaction; lane 6, HDAC1 complex with wild type HDAC1 in the reaction. C, model of active and inactive HDAC1/2-containing complexes. Acetylation on HDAC1 inhibits the deacetylase activity of both HDAC1 and HDAC2, resulting in the inactivation of corepressor complexes.