FIGURE 1.

Physical map of the pycA, pycB, and fumA genes in P. fluorescens CHA0 and construction of strains CHA1321 (pycA) and CHA1322 (fumA). ▾, Tn5 insertion in the pycB gene of strain CHA1201; Ω, region replaced by the ΩSp/Sm cassette in strain CHA1321; ▵, region deleted in the fumA of strain CHA1322. The open boxes below indicate the regions used for complementation, either in mini-Tn7 (via pME9951 and pME9955) or in the pME6031 derivatives pME9952 and pME9956, as described under “Experimental Procedures.” PFL gene numbers refer to the genome of P. fluorescens Pf-5 (15).