TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| M168 | Wild type | C. Keel |
| E. coli | ||
| DH5α, HB101 | Laboratory strains | Ref. 37 |
| S17-1/λpir | pro thi hsdR recA chromosome::RP4–2 (Tc::Mu Km::Tn7) λpir; Tpr Smr | Ref. 38 |
| P. aeruginosa | ||
| PAO1 | Wild type | ATCC15692 |
| PAO6382 | ΔpvdF | C. Reimmann |
| P. fluorescens | ||
| CHA0 | Wild type | Ref. 39 |
| CHA19 | ΔgacS | Ref. 40 |
| CHA89 | gacA::Kmr | Ref. 41 |
| CHA1201 | pycB::Tn5, insertion 1.6 kb from 5′-end | This study |
| CHA1201C | pycB::Tn5 strain with pycAB+ region in mini-Tn7 | This study |
| CHA1201Cp | pycB::Tn5 strain harboring pME9952 | This study |
| CHA1202 | ΔretS | Ref. 30 |
| CHA1204 | ΔladS | Ref. 30 |
| CHA1313 | ΔgacS pycA::ΩSp/Sm | This study |
| CHA1314 | gacA::KmrpycA::ΩSp/Sm | This study |
| CHA1315 | ΔretS pycA::ΩSp/Sm | This study |
| CHA1316 | ΔladS pycA::ΩSp/Sm | This study |
| CHA1317 | ΔgacS ΔfumA | This study |
| CHA1318 | gacA::Kmr ΔfumA | This study |
| CHA1319 | ΔretS ΔfumA | This study |
| CHA1320 | ΔladS ΔfumA | This study |
| CHA1321 | pycA::ΩSp/Sm | This study |
| CHA1322 | ΔfumA | This study |
| CHA1322C | ΔfumA strain with fumA+ region in mini-Tn7 | This study |
| CHA1322Cp | ΔfumA strain harboring pME9956 | This study |
| Plasmids | ||
| pBluescript II KS | Cloning vector, ColE1 replicon; Apr | Stratagene |
| pHP45Ω | Source of the ΩSp/Smr cassette, ColE1 vector; Apr | Ref. 29 |
| pLM1 | Tn5Gm delivery vector, Gmr derivative of pRL27 | Ref. 27 |
| pME497 | Mobilizing plasmid, IncP-1, Tra, RepA(Ts); Apr | Ref. 42 |
| pME3087 | Suicide vector, ColE1 replicon, Mob; Tcr | Ref. 43 |
| pME3266 | Translational gacS′-′lacZ fusion; Tcr | F. Carruthers |
| pME6031 | pACYC177-pVS1 shuttle vector; Tcr | Ref. 44 |
| pME6060 | Translational aprA'-'lacZ fusion; Tcr | Ref. 45 |
| pME6091 | Transcriptional rsmZ-lacZ fusion; Tcr | Ref. 11 |
| pME6182 | Mini-Tn7 gene delivery vector based on pME3280a, HindIII-SmaI-KpnI-NcoI-SphI cloning site, ColE1 replicon; Gmr Apr | Ref. 30 |
| pME6702 | Translational phlA′-′lacZ fusion under ptac; Tcr | Ref. 17 |
| pME6827 | Translational gacA′-′lacZ fusion; Tcr | C. Reimmann |
| pME6916 | Transcriptional rsmY-lacZ fusion; Tcr | Ref. 16 |
| pME7402 | Transcriptional rsmZ-gfp fusion; Tcr | Ref. 21 |
| pME7415 | Transcriptional rsmY-gfp fusion; Tcr | This study |
| pME7698 | pME6182 containing a transcriptional rsmX-lacZ fusion in mini-Tn7 | B. Humair |
| pME7699 | pME6182 containing a transcriptional rsmY-lacZ fusion in mini-Tn7 | B. Humair |
| pME9951 | pME6182 containing the pycAB genes in mini-Tn7 | This study |
| pME9952 | pME6031 containing the pycAB genes | This study |
| pME9953 | pME3087 containing pycAB region with a 0.5-kb EcoRV deletion in pycA replaced by the 2-kb ΩSp/Sm fragment | This study |
| pME9954 | pME3087 containing a 1.4-kb fumA region with a 0.9-kb deletion in the fumA gene | This study |
| pME9955 | pME6182 containing the fumA gene in mini-Tn7 | This study |
| pME9956 | pME6031 containing the fumA gene | This study |
| pPROBE-TT′ | gfp promoter probe vector; Tcr | Ref. 46 |
| pUX-BF13 | Helper plasmid encoding Tn7 transposition fusions; Apr | Ref. 47 |