TABLE 3.
Intracellular concentrations of central metabolites determined in cell extracts of P. fluorescens strains CHA0, CHA1322 (fumA−), and CHA1201 (pycB−) during exponential growth (trophophase) and postexponential growth phase (idiophase).
| Metabolite | Phasea | Concentration (μmol/g) |
||
|---|---|---|---|---|
| Wild type | fumA− | pycB− | ||
| μ mol/g | ||||
| Hexose-P | T | 5.4 ± 1.0 | 4.6 ± 0.3 | 4.2 ± 0.3 |
| I | 2.0 ± 0.1 | 2.5 ± 0.5 | 1.7 ± 0.1 | |
| 3-Phosphoglycerate | T | 1.5 ± 0.3 | 1.1 ± 0.1 | 1.1 ± 0.1 |
| I | 0.6 ± 0.1 | 0.7 ± 0.2 | 0.9 ± 0.5 | |
| Phosphoenolpyruvate | T | 1.5 ± 0.2 | 0.8 ± 0.1 | 1.2 ± 0.1 |
| I | 0.4 ± 0.1 | 0.6 ± 0.1 | 0.5 ± 0.1 | |
| Pyruvate | T | 9.2 ± 4.9 | 5.7 ± 0.4 | 20b ± 1 |
| I | 2.1 ± 0.2 | 4.5 ± 0.5 | 1.8 ± 0.4 | |
| Citrate + isocitratec | T | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.1 ± 0.1 |
| I | 1.6 ± 0.5 | 1.9 ± 0.3 | 0.8 ± 0.2 | |
| Succinate | T | 7.1 ± 4.4 | 29b ± 5 | 6.4 ± 3.0 |
| I | 4.1 ± 3.3 | 12b ± 3 | 0.9 ± 0.4 | |
| Fumarate | T | 0.4 ± 0.05 | 140b ± 7 | 0.5 ± 0.05 |
| I | 0.2 ± 0.05 | 62b ± 16 | 0.2 ± 0.05 | |
| Malate | T | 0.1 ± 0.05 | 0.3 ± 0.05 | 0.3 ± 0.05 |
| I | <0.1 ± 0.05 | 0.2 ± 0.05 | <0.05 ± 0.05 | |
| 2-Oxoglutarate | T | 2.3 ± 0.1 | 10 ± 0.6 | 1.8 ± 0.05 |
| I | 0.4 ± 0.05 | 0.5 ± 0.1 | 0.1 ± 0.05 | |
a T, trophophase; I, idiophase.
b Values outside linear range.
c Metabolites not separated.