FIGURE 6.

TcUBP1 RRM couples arsenite-induced nuclear accumulation, mRNA granule association, and RNA binding capacity. A scheme of the domains (not to scale) of the most relevant constructs used for determining the sequences required for nuclear shuttling are shown. Coverage is shown in parentheses and mutated residues are shown in brackets. Localization is referred to untreated parasites grown in culture. Arsenite-induced nuclear localization, mRNA granules localization, and RNA binding in vitro are also summarized. C, cytoplasmic, C > N, more cytoplasmic than nuclear; C = N, even in cytoplasm and nucleus. Asterisk (*) is referred to the RNA binding capacity of the protein without the β-Gal moiety. Double asterisk (**) is referred to the RNA binding capacity of the protein without the NLS. N.D., not determined. n/a, not applicable.