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. 2009 Sep 29;284(50):35079–35091. doi: 10.1074/jbc.M109.040774

FIGURE 1.

FIGURE 1.

Genetic network between LTV1, PRP43, PFA1, and NOB1. A, identification of PRP43, PFA1, and NOB1 in a synthetic lethal screen with an ltv1 deletion strain. Mutants SL179 and SL181 isolated in the synthetic lethal (sl) screen were transformed with plasmids carrying the indicated genes and spotted in serial 10-fold dilution steps onto SDC−leu and SDC + 5-fluoroorotic acid (5-FOA) plates. As control, the non-mutagenized screening strain (ltv1Δ, pHT4467-CENΔ-LTV1) was spotted. Plates were incubated at 30 °C for 4 days. Red colony color on SDC−leu and slow growth on 5-fluoroorotic acid plates indicate a synthetic growth defect. Red/white sectoring on SDC−leu and growth on 5-fluoroorotic acid indicate complementation of the synthetic enhancement phenotype. B, temperature dependence of genetic interactions between LTV1 and PRP43 and between LTV1 and PFA1. The LTV1/PFA1 and LTV1/PRP43 shuffle strains were transformed with plasmids that carry the indicated wild-type and mutant alleles. pfa1ΔG is a PFA1 allele lacking the G-patch. After 5-fluoroorotic acid shuffling, cells were spotted in 10-fold serial dilution steps onto SDC−leu−trp plates to select for the transformed plasmids and incubated at 23, 30, and 37 °C for 4 days.