TABLE 1.
Proteins modulated by miR-210
| Gene | Description | Spot | MW kDa/pIa | Fold increaseb | S.E. | p |
|---|---|---|---|---|---|---|
| Up-regulated | ||||||
| CNN2 | Calponin-2 | 34 | 33.7/6.94 | 2.15 | 0.12 | 0.002 |
| EHD1 | EH-domain-containing protein 1 | 62 | 60.6/6.35 | 1.75 | 0.07 | 0.002 |
| EIF5A | Eukaryotic translation initiation factor 5A | 6 | 16.8/5.07 | 3.29 | 0.34 | 0.008 |
| GIPC1 | RGS19-interacting protein 1 | 75 | 36.0/5.90 | 1.64 | 0.11 | 0.037 |
| HNRPL | Heterogeneous nuclear ribonucleoprotein L | 91 | 64.1/8.46 | 1.52 | 0.07 | 0.035 |
| HSPD1 | 60 kDa heat shock protein | 70 | 61.1/5.70 | 1.69 | 0.15 | 0.030 |
| KHSRP | Far upstream element-binding protein 2 | 15 | 73.1/6.84 | 2.69 | 0.30 | 0.009 |
| KHSRP | Far upstream element-binding protein 2 | 81 | 73.1/6.84 | 1.59 | 0.05 | 0.041 |
| LMNA | Lamin A/C | 1 | 74.1/6.57 | 5.14 | 0.60 | 0.007 |
| LMNA | Lamin A/C | 39 | 74.1/6.57 | 2.04 | 0.21 | 0.026 |
| LOC403161 | Nicotinate phosphoribosyltransferase-like protein | 58 | 57.5/5.51 | 1.80 | 0.12 | 0.007 |
| MCCC2 | Methylcrotonoyl-CoA carboxylase β-chain | 53 | 61.3/7.58 | 1.85 | 0.24 | 0.046 |
| MTHFD1 | C-1-tetrahydrofolate synthase | 21 | 101.5/6.89 | 2.59 | 0.27 | 0.012 |
| NUP54 | Nucleoporin 54 kDa | 87 | 54/6.53 | 1.54 | 0.10 | 0.030 |
| PCBP1 | Poly(rC)-binding protein 1 | 92 | 37.5/6.66 | 1.51 | 0.03 | 0.002 |
| PDHA1 | Pyruvate dehydrogenase E1 component α-subunit | 100 | 43.3/8.35 | 1.43 | 0.11 | 0.036 |
| SARS | Seryl-tRNA synthetase | 68 | 58.2/8.35 | 1.71 | 0.14 | 0.020 |
| TUBA3 | Tubulin α-3 chain | 87 | 49.9/4.96 | 1.54 | 0.10 | 0.030 |
| UCHL1 | Ubiquitin carboxyl-terminal hydrolase isozyme L1 | 79 | 24.8/5.33 | 1.61 | 0.16 | 0.043 |
| Down-regulated | ||||||
| BCAS2 | Breast carcinoma amplified sequence 2 | 41 | 26.1/5.48 | 0.50 | 0.06 | 0.023 |
| CBX1 | Chromobox protein homolog 1 | 28 | 21.4/4.85 | 0.44 | 0.06 | 0.020 |
| CTSB | Cathepsin B precursor | 60 | 37.8/5.88 | 0.57 | 0.06 | 0.029 |
| CTSB | Cathepsin B precursor | 69 | 37.8/5.88 | 0.59 | 0.05 | 0.024 |
| EIF1AY | Eukaryotic translation initiation factor 1A. Y-chromosomal | 97 | 16.5/5.07 | 0.68 | 0.05 | 0.031 |
| NDUFV2 | NADH-ubiquinone oxidoreductase 24 kDa subunit | 80 | 27.4/8.22 | 0.62 | 0.05 | 0.040 |
| NES | Nestin | 36 | 177.4/4.35 | 0.47 | 0.06 | 0.028 |
| P4HB | Protein disulfide-isomerase precursor | 17 | 57.1/4.76 | 0.38 | 0.07 | 0.030 |
| PTPN1 | Tyrosine-protein phosphatase non-receptor type 1 | 72 | 49.9/5.88 | 0.60 | 0.06 | 0.035 |
| SEC13L1 | SEC13-related protein | 29 | 35.5/5.22 | 0.45 | 0.07 | 0.038 |
| TCEB2 | Transcription elongation factor B polypeptide 2 | 78 | 13.3/4.73 | 0.62 | 0.04 | 0.033 |
| VIM | Vimentin | 10 | 53.6/5.06 | 0.33 | 0.07 | 0.029 |
| VIM | Vimentin | 2 | 53.6/5.06 | 0.21 | 0.01 | 0.000 |
| VIM | Vimentin | 12 | 53.6/5.06 | 0.37 | 0.06 | 0.045 |
a kDa/isoelectric point.
b Ratio of normalized spot density from cells overexpressing miR-210 versus control cells.