TABLE 1.
Oligodeoxynucleotides used in analyses
X indicates A, C, G, or T; Xdd indicates a terminal 2′,3′-dideoxy-X (incapable of phosphodiester bond formation); YYY indicates GGC, GGT, AAC, CAG, GAG, GAC, TTC, GTC, TCG, CCT, GCT, GCA, G(O6-MeG)C, G(8-oxoG)C, G(1,N2-ϵ-G)C, G(1,N2-ϵ-G)T, or G(AP)C. The primer terminal residue X of the 13-mer primer was paired with the first (3′) Y in the YYY sequence of the template strand. For the LC-MS experiments, the 5′-most T of the primer was replaced with 2′-deoxyuridine (see Table 2).
| 13-mer | 5′-GGGGGAAGGATTX-3′ |
| 13dd-mer | 5′-GGGGGAAGGATTXdd-3′ |
| 18-mer | 3′-CCCCCTTCCTAAYYYACT-5′ |