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. 2009 Sep 2;284(50):35189–35200. doi: 10.1074/jbc.M109.053835

FIGURE 1.

FIGURE 1.

Structure-based multiple sequence alignment of the sulfatases using ClustalW. The flavobacterial N-sulfamidase enzyme is a member of a large sulfatase family. The putative active site is boxed, with the critically modified cysteine 80 noted by an asterisk. Invariant residues are shaded in dark gray; those with partial identity in light gray, and conservative substitutions in charcoal. Multiple sequence alignment was generated by ClustalW using only select sequences identified from a BLASTP search of the protein data base. Enzymes with the following GenBankTM accession numbers are abbreviated as follows: F. heparinum N-sulfamidase (Nsulf); F. heparinum 6-O-sulfatase (FH6S); F. heparinum 2-O-sulfatase (FH2S); P. aeruginosa arylsulfatase (PARS, GenBankTM code CAA88421); human N-acetylgalactosamine-6-sulfate sulfatase or chondroitin-6-sulfatase (HGal6S, GenBankTM code AAC51350); human cerebroside-3-sulfate sulfatase or arylsulfatase A (HARSA, GenBankTM code AAC51350); human N-acetylgalactosamine-4 sulfate sulfatase or arylsulfatase B (HARSB, GenBankTM code AAC51350).