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Proposed mechanism for cleavage of nitrogen-sulfur bond by N-sulfamidase. The color coding of the amino acids is as mentioned in Fig. 3 legend. The mechanism is depicted in parts for clarity, and all interactions are shown as broken lines. Transfer of protons and electrons are shown by arrows to denote directionality of transfer. The alternate pathway where Lys-160 potentially mediates the sulfate transfer to the formylglycine is shown with dotted arrow.
