FIGURE 3.

Interaction of CI-MPR constructs with the phosphorylated glycan microarray. The phosphorylated glycan microarray was printed as described in the accompanying article (64) and the individual glycans are identified by their glycan number (glycan number) as indicated in Table 1 of the accompanying article (64). Glycans 1–8 are non-phosphorylated high mannose-type oligosaccharides; 9–16, phosphodiesters of high mannose-type oligosaccharides; and 17–24, phosphomonoesters of high mannose-type oligosaccharides. The structural assignments are made according to Table 1 in the accompanying article (64) and are shown in Fig. 3. CI-MPR constructs were applied to the array at 50 μg/ml and detected with polyclonal rabbit antibody 14.5 as described under “Experimental Procedures.” A, sCI-MPR is the same data shown in Fig. 4D of the accompanying article (64); B, Dom1–3; C, Dom5; D, Dom5–9; E, Dom7–11; and F, Dom9. Error bars represent the mean ± S.D. of 6 replicates after removing the high and low values.