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. 2009 Oct 19;284(50):35215–35226. doi: 10.1074/jbc.M109.056184

FIGURE 5.

FIGURE 5.

Inhibition of Dom5His and Dom5–9 binding to GAA by Man-6-P, Glc-6-P, and N-acetylglucosaminyl 6-phosphomethylmannoside diester. Aliquots of 500 nm Dom5His (A) and 50 nm Dom5–9 (B) were incubated with increasing concentrations of Glc-6-P (▿), Man-6-P (●), or N-acetylglucosaminyl 6-phosphomethylmannoside diester (○) and injected over a flow cell coupled with GAA diester. The Req from the resulting sensorgrams was plotted as a percent of the maximal response against the log of the inhibitor concentration. The Ki was determined using nonlinear regression (SigmaPlot, version 10.0).