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. 2009 Oct 19;284(50):35215–35226. doi: 10.1074/jbc.M109.056184

TABLE 1.

Estimated, relative binding affinities of CI-MPR and truncated forms of sCI-MPR based on half-maximal binding to the phosphorylated glycan microarray

Glycan No. Designation CI-MPRa Dom1–3-His Dom5-His Dom5–9 Dom9His Dom7–11
9 GPM6 6.2 × 10−10 DNBb 8.7 × 10−6 3.7 × 10−9 DNB DNB
10 2(GP)M6 8.0 × 10−11 DNB 6.9 × 10−6 3.3 × 10−9 1.1 × 10−6 5.48 × 10−7
11 GPM7(3) 5.9 × 10−9 DNB 1.8 × 10−5 8.9 × 10−9 DNB DNB
12 GPM7(2) >5.8 × 10−8 DNB DNB >2.8 × 10−7 DNB DNB
13 2(GP)M7(1)+(3) 6.0 × 10−11 DNB 4.3 × 10−6 3.0 × 10−9 DNB DNB
14 GPM8(1) 1.1 × 10−10 DNB 1.6 × 10−5 4.0 × 10−9 DNB DNB
15 GPM9 1.9 × 10−10 DNB 1.8 × 10−5 5.7 × 10−9 DNB DNB
16 2(GP)M9 7.0 × 10−11 DNB 4.8 × 10−6 4.1 × 10−9 DNB DNB
17 PM6 7.0 × 10−11 1.1 × 10−7 DNB 1.4 × 10−8 6.6 × 10−8 5.5 × 10−8
18 2(P)M6 8.0 × 10−11 1.0 × 10−7 DNB 1.9 × 10−8 5.7 × 10−8 6.7 × 10−8
19 PM7(3) 9.0 × 10−11 1.3 × 10−7 DNB 2.5 × 10−8 6.8 × 10−8 8.1 × 10−8
20 PM7(2) 5.2 × 10−9 >1.0 × 10−6 DNB >2.8 × 10−7 >1.2 × 10−6 >6.0 × 10−7
21 2(P)M7(1)+(3) 8.0 × 10−11 8.7 × 10−8 DNB 1.7 × 10−8 5.5 × 10−8 4.8 × 10−8
22 PM8(1) 1.7 × 10−10 >1.0 × 10−6 DNB 4.2 × 10−8 1.9 × 10−7 2.6 × 10−7
23 PM9 1.2 × 10−10 >1.0 × 10−6 DNB 5.7 × 10−8 1.5 × 10−7 2.7 × 10−7
24 2(P)M9 8.0 × 10−11 1.4 × 10−7 DNB 2.6 × 10−8 5.2 × 10−8 8.6 × 10−8

a The half-maximal binding concentration (μg/ml) for each MPR construct was estimated from graphs of the data shown in supplemental Fig. S1. The estimated Kd value are shown in molar concentrations using the following molecular mass assignments: sCI-MPR (dimer), 520,000 Da; Dom1–3His, 50,000 Da; Dom5His, 21,000 Da; Dom5–9, 90,000 Da; Dom9His (dimer), 42,000 Da; and Dom7–11, 84,000 Da.

b DNB, did not bind.