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Spectra of SDF-1α with an excess amount of CXCR4 and CXCR4-AMD3100. A–C, lower panels show the two-dimensional 1H-13C HMQC spectra of 10 μm [[U-2H]Leu-,Val-13C1H3,12C2H3)]SDF-1α in the free state (A), with an excess amount (20 μm) of CXCR4 (B), and with an excess amount (20 μm) of CXCR4 and 1 mm AMD3100 (C). The upper panels show the 1H one-dimensional slice in each 1H-13C HMQC spectrum at the corresponding 13C frequencies indicated with lines in the two-dimensional spectra.
