Figure 8.—

PGL-1 accumulates in numerous large granules in the germ line after an endogenous siRNA pathway is compromised. (A) Endogenous PGL-1 staining (green) resembles wild type in the germ lines of rde-1, rrf-1, and rrf-3 mutants. Large PGL-1 granules accumulate in the germ lines of ego-1 and csr-1 mutants. Most granules are dissociated from the nuclear periphery. (B) GFP∷PGL-1 accumulation in control(RNAi), drh-3(RNAi), and ego-1(RNAi) germ lines. RNAi depletion of EGO-1 and DRH-3 produces the same large PGL-1 granule phenotype as depletion of CSR-1. Bars, 10 μm.