TABLE 1.
GFP∷PGL-1 screen positives
| Gene name | Wormbase ID | Gene class codec | GFP∷PGL-1 in the P0 germ line |
GFP∷PGL-1 in F1 embryos |
PGL-1 phenotype preceded by general embryo defects? | Endogenous PGL-1 |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| Description | Altered level | Altered pattern | Altered leveld | Altered pattern | Stained | Same as GFP | ||||
| adr-1 | H15N14.1 | RNA adenosine deaminase | Abnormal | Yes | ||||||
| air-1 | K07C11.2 | Aurora kinase | C | Low | Diffuse | Yes | ||||
| apc-2 | K06H7.6 | Anaphase-promoting complex 2 | U | Low | Diffuse | Yes | ||||
| arl-1 | F54C9.10 | GTP-binding ADP-ribosylation factor | G | Diffuse | Yes | |||||
| atx-2 | D2045.1a | Ataxin-related protein | Low | Diffuse | No | X | X | |||
| bir-1 | T27F2.3 | Aurora B kinase complex | C | Low | No | X | X | |||
| cct-2 | T21B10.7 | Cytosolic “T complex” chaperonin | T | Diffuse | No | X | X | |||
| cct-3 | F54A3.3 | Cytosolic T complex chaperonin | T | Low | Diffuse | Yes | ||||
| cct-7 | T10B5.5 | Cytosolic T complex chaperonin | T | Low | Yes | |||||
| cct-8 | Y55F3AR.3ab | Cytosolic T complex chaperonin | T | Low | Diffuse | Yes | X | X | ||
| cdc-25.1 | K06A5.7 | Cell division cycle 25.1 phosphatase | C | Low | Diffuse. Puncta in multiple cells | No | X | X | ||
| cdc-42 | R07G3.1 | Cell division cycle 42 Rho GTPase | C | Low | Diffuse | No | X | |||
| cdk-1 | T05G5.3 | Cyclin-dependent kinase 1 | C | Low | Diffuse | Yes | ||||
| cdk-9 | H25P06.2 | Cyclin-dependent kinase 9 | C | Low or und. | Yes | X | X | |||
| ceh-21 | T26C11.6 | ONECUT class homeobox protein | Low | No | X | |||||
| cgh-1 | C07H6.5 | Germ-line DEAD-box RNA helicase | Low | No | X | X | ||||
| cpf-2 | F56A8.6 | Cleavage and polyadenylation factor | S | Low | Diffuse in P cells | No | X | |||
| cpl-1 | T03E6.7 | Cathepsin L-like cysteine protease 1 | Low | Diffuse | Yes | |||||
| csr-1 | F20D12.1 | Secondary RNA slicing Argonaute | High | Large granules | High | No | X | X | ||
| cyb-3 | T06E6.2 | Cyclin B3 protein | Low | Diffuse | Yes | |||||
| cyk-4 | K08E3.6 | Rho GTPase-activating protein (GAP) | C | Und. | Yes | |||||
| cyp-31A3 | Y17G9B.3 | CYP7B1 homolog —vitamin D deficiency | Low | Abnormal | No | X | X | |||
| dhc-1 | T21E12.4 | Dynein heavy chain | C | Diffuse | Yes | |||||
| dlc-1 | T26A5.9 | Dynein light chain | N | Low | Yes | |||||
| dnc-1 | ZK593.5 | Dynactin/GLUED orthologs | C | Low | Yes | X | X | |||
| dom-6 | C09G4.3 | Cks/Suc1 ortholog. promotes cyclin B degradation | C | Low | Diffuse | Yes | X | X | ||
| dpf-1 | T23F1.7 | Prolyl oligopeptidase | Low | Diffuse | No | X | ||||
| dyci-1 | C17H12.1 | Dynein cytoplasmic intermediate chain | Diffuse | Yes | ||||||
| eft-1 | ZK328.2 | Tu domain containing elongation factor | High | Diffuse | High in P cells | Diffuse in soma | No | X | X | |
| egg-3 | F44F4.2 | Protein–tyrosine phosphatase | C | Low | Diffuse | Yes | ||||
| egl-45 | C27D11.1 | Eukaryotic translation initiation factor 3 subunit 10 | R | Low or und. | No | X | X | |||
| eif-3.F | D2013.7 | Eukaryotic translation initiation factor 3 subunit 5 | R | Low | No | X | X | |||
| elc-1 | Y82E9BR.15 | Elongin C | U | Undetectable | Und. | Yes | X | X | ||
| emb-9 | K04H4.1 | Collagen | Low | No | X | |||||
| fbxa-81 | T24C2.4 | F-box domain | U | Low | No | X | ||||
| fbxb-51 | K05F6.3 | F-box domain | U | High | High in P cells | Large granules | No | X | X | |
| fbxb-60 | F55C9.7 | F-box domain | U | High | No | X | ||||
| flp-13 | F33D4.3 | FMRF-like amide neurotransmitter | High | High in P cells | Diffuse in soma | No | X | |||
| glb-27 | W01C9.5 | Unknown | Low | No | X | |||||
| gon-1 | F25H8.3 | Secreted metalloprotease (MPT) | Low | Diffuse | No | X | X | |||
| gsk-3 | Y18D10A.5 | Glycogen synthase kinase required for polarization of EMS and P2 blastomeres | C | Low | Diffuse in multiple cells | Yes | ||||
| his-40 | K03A1.1 | Similarity to histones (pseudogene?) | Diffuse in P cells | Yes | ||||||
| his-48 | B0035.8 | H2B histone | Low | Yes | ||||||
| hsp-6 | C37H5.8 | HSP70-like mitochondrial chaperone | Low | No | X | |||||
| ify-1 | C27A2.3 | Ligand of Fizzy protein. Securin? | C | Diffuse | Yes | |||||
| imb-4 | ZK742.1 | Exportin-1/CRM1 nuclear exportin | N | Diffuse in P cells | No | X | X | |||
| imb-5 | Y48G1A.5ab | CSE1/CAS-like nuclear importin | N | Diffuse in P cells | No | X | X | |||
| inx-8 | ZK792.2 | Innexin/GAP junction component | Low | No | X | |||||
| let-70 | M7.1 | E2 ubiquitin-conjugating enzyme | U | Low | Diffuse | Yes | ||||
| let-711 | F57B9.2 | NOT1 deadenylation component | High | Diffuse | High | No | X | X | ||
| let-92 | F38H4.9 | Catalytic subunit of PP2A phosphatase | Diffuse | Yes | X | X | ||||
| let-99 | K08E7.3 | DEP domain. Spindle orientation | C | Low | No | X | X | |||
| lin-41 | C12C8.3 | RING finger B box coiled-coil protein | Low in some | No | X | |||||
| lmn-1 | DY3.2 | Nuclear type B lamin | N | Diffuse in P cells | No | X | X | |||
| mag-1 | R09B3.5 | Mago nashi. Exon–exon junction factor | S | Low in some | No | X | ||||
| mbk-2 | F49E11.1 | Minibrain DYRK2 kinase | C | Low | No | X | X | |||
| mcm-2 | Y17G7B.5 | MCM licensing factor 2 | M | Low | Diffuse in P cells | Yes | X | X | ||
| mcm-4 | Y39G10AR.14 | MCM licensing factor 4 | M | Low | Diffuse in P cells, puncta in soma | Yes | X | X | ||
| mcm-5 | R10E4.4 | MCM licensing factor 5 | M | Diffuse in P cells | Yes | X | X | |||
| mcm-6 | ZK632.1 | MCM licensing factor 6 | M | Diffuse in P cells, high puncta in soma | Yes | X | X | |||
| mcm-7 | F32D1.10 | MCM licensing factor 7 | M | Diffuse in P cells | Yes | X | X | |||
| mdt-6 | Y57E12AL.5 | Transcriptioinal mediator Med6-like | Low | Diffuse | Yes | |||||
| mel-26 | ZK858.4 | Adaptor of the E3 ubiquitin ligase | U | Diffuse | Yes | |||||
| mix-1 | M106.1 | SMC2-like condensing complex factor | Low | No | X | |||||
| nmy-2 | F20G4.3 | Nonmuscle myosin II | C | Low | Diffuse | Low | Diffuse | Yes | X | X |
| npp-10 | ZK328.5 | NUP98-like nucleoporin | N | Diffuse in P cells | No | X | X | |||
| npp-20 | Y77E11A.13 | Nucleoporin | N | Low | Diffuse | Yes | X | X | ||
| npp-6 | F56A3.3 | NUP160-like nucleoporin | N | Diffuse in P cells | No | X | X | |||
| npp-7 | T19B4.2 | Nucleoporin | N | Diffuse in P cells | No | X | X | |||
| npp-9 | F59A2.1 | Nucleoporin | N | Diffuse in P cells | No | X | X | |||
| paa-1 | F48E8.5 | Regulatory subunit of PP2A phosphatase | Diffuse | Yes | X | X | ||||
| par-1 | H39E23.1 | Serine/threonine kinase | C | Low or und. | Diffuse | No | X | X | ||
| par-2 | F58B6.3 | C3HC4-type RING-finger protein | C | Low or und. | Yes | |||||
| par-5 | M117.2 | 14-3-3-containing protein | C | Low | Diffuse | No | X | X | ||
| par-6 | T26E3.3 | PDZ-domain-containing protein | C | Low or und. | Diffuse | Yes | ||||
| pas-4 | C36B1.4 | α4 20S proteasome core subunit | P | Low or und. | Yes | X | X | |||
| pas-5 | F25H2.9 | α5 20S proteasome core subunit | P | Low or und. | Yes | |||||
| pbs-1 | K08D12.1 | β1 20S proteasome core subunit | P | Low | Diffuse | Yes | X | X | ||
| pbs-3 | Y38A8.2 | β3 20S proteasome core subunit | P | Und. in some | Yes | |||||
| pbs-4 | T20F5.2 | β4 20S proteasome core subunit | P | Diffuse | Yes | |||||
| pbs-5 | K05C4.1 | β5 20S proteasome core subunit | P | Diffuse | Low | Diffuse | Yes | X | X | |
| pbs-6 | C02F5.9 | β6 20S proteasome core subunit | P | Diffuse | Yes | |||||
| pbs-7 | F39H11.5 | β7 20S proteasome core subunit | P | Diffuse | Yes | |||||
| pgl-1 | ZK381.4 | RGG box motifs. | Und. | No | ||||||
| phi-10 | Y46G5A.4 | DEAD-box RNA helicase BRR2 | S | High | High | No | X | X | ||
| phi-12 | M03F8.3 | HAT repeat. mRNA splicing | S | Low | No | X | X | |||
| phi-21 | T05H4.6 | Translation release factor | Low | Diffuse | No | X | X | |||
| phi-6 | K02F2.3 | snRNP-associated factor | S | High | High in P cells | Diffuse in soma | No | X | X | |
| phi-62 | F49C12.12 | Unknown | Low | No? | X | X | ||||
| phi-7 | D1081.8 | mRNA splicing protein CDC5 (Myb) | S | High | Diffuse | High in P cells | No | X | X | |
| plk-1 | C14B9.4 | Serine/threonine polo-like kinase | C | Low | Diffuse | Yes | X | X | ||
| pri-1 | F58A4.4 | DNA Pol α-primase subunit D | Low | No | X | X | ||||
| prp-8 | C50C3.6 | U5 snRNA-associated splicing factor | S | High | Diffuse | High | Diffuse | No | X | X |
| prs-1 | T20H4.3 | Proline tRNA ligase | Low | No? | X | X | ||||
| puf-3 | Y45F10A.2 | PUF family RNA-binding protein | Diffuse | No | X | X | ||||
| ran-1 | K01G5.4 | RAN-associated GTP-binding protein | N | Low | Diffuse | No | X | X | ||
| ran-4 | R05D11.3 | Nuclear transport factor 2 (NTF2) | N | Low | Diffuse in P cells | No | X | X | ||
| rnp-4 | R07E5.14 | Exon–exon junction complex factor | S | Diffuse in P cells | Yes | |||||
| rnr-1 | T23G5.1 | Ribonucleotide reductase | Low or und. | Diffuse in P cells | No | X | X | |||
| rnr-2 | C03C10.3 | Ribonucleotide reductase | Diffuse in P cells | No | X | X | ||||
| rpa-1 | F18A1.5 | Large subunit of replication protein A | Diffuse in P cells, high puncta in soma | Yes | ||||||
| rpb-2 | C26E6.4 | RNA Pol II β-subunit | Diffuse in P cells | Yes | X | X | ||||
| rpl-12 | JC8.3 | Ribosomal protein large subunit 12 | R | Low | No | X | ||||
| rpl-18 | Y45F10D.12 | Ribosomal protein large subunit 18 | R | Low | Yes | X | X | |||
| rpl-20 | E04A4.8 | Ribosomal protein large subunit 18a | R | Low | No | X | X | |||
| rpl-36 | F37C12.4 | Ribosomal protein large subunit 36 | R | Low | No | X | X | |||
| rpn-1 | T22D1.9a | Proteasome regulatory particle | P | Diffuse | Yes | |||||
| rpn-11 | K07D4.3 | Proteasome regulatory particle | P | Diffuse in P cells | Yes | |||||
| rpn-3 | C30C11.2 | Proteasome regulatory particle | P | Diffuse | Yes | |||||
| rps-0 | B0393.1 | Ribosomal protein small subunit 2A | R | Low | No | X | X | |||
| rps-20 | Y105E8A.16 | Ribosomal protein small subunit 20 | R | Low | Yes | |||||
| rps-22 | F53A3.3 | Ribosomal protein small subunit 15a | R | Low | No | X | X | |||
| rps-24 | T07A9.11 | Ribosomal protein small subunit 24 | R | Low | No | X | ||||
| rps-3 | C23G10.3a | Ribosomal protein small subunit 3 | R | Low | No | X | ||||
| rps-5 | T05E11.1 | Ribosomal protein small subunit 5 | R | Low | No | X | ||||
| rps-8 | F42C5.8b | Ribosomal protein small subunit 8 | R | Low | Diffuse | Yes | ||||
| rps-9 | F40F8.10 | Ribosomal protein small subunit 9 | R | Low | No | X | X | |||
| rpt-1 | C52E4.4 | Proteasome regulatory particle | P | Low | Diffuse | Yes | ||||
| rpt-3 | F23F12.6 | Proteasome regulatory particle | P | Diffuse | Yes | |||||
| rpt-5 | F56H1.4 | Proteasome regulatory particle | P | Diffuse | Yes | |||||
| rsp-7 | D2089.1 | SR protein (splicing factor) 7 | S | Low | Diffuse in P cells | No | X | X | ||
| ruvb-1 | C27H6.2 | RuvB-like DNA helicase | Low | No | X | |||||
| ruvb-2 | T22D1.10 | RuvB-like DNA helicase | Low | No | X | |||||
| scp-1 | D2013.8 | WD repeat, sreb cleavage protein | G | Low in some | No | X | ||||
| skp-1 | T27F2.1 | SKI-binding protein (SKIP) orthologs | S | High | High | Diffuse in soma | No | X | X | |
| snr-2 | W08E3.1 | Core splicesome component | S | Low | Yes | |||||
| spd-5 | F56A3.4 | Dynein heavy chain 1 | C | Diffuse | Yes | |||||
| sqv-6 | Y50D4C.4 | Xylosyltransferase | C | Low or und. | Yes | |||||
| srh-81 | W03F9.7 | Serpentine receptor class H | Low | No | X | |||||
| sri-25 | C41G6.10 | Serpentine receptor class I | Low | No | X | |||||
| srj-6 | F31F4.8 | Serpentine receptor class J | Low | No | X | |||||
| sru-41 | Y51H7BR.6 | Serpentine receptor class U | Low | No | X | |||||
| srx-38 | C03A7.5 | Serpentine receptor class X | Low | No | X | |||||
| tag-198 | F09G8.2 | Putative DNase II | Low | No | X | |||||
| tag-49 | AC7.1 | G-protein receptor | Low | No | X | X | ||||
| tbb-2 | C36E8.5 | β-Tubulin | Diffuse | Yes | X | X | ||||
| tbg-1 | F58A4.8 | γ-Tubulin | C | Low | Yes | |||||
| ttr-14 | T05A10.3 | Transthyretin-related family domain | Low | No | X | |||||
| uba-1 | C47E12.5 | Ubiquitin UBA1/UBE1 related | U | Low | Diffuse | Yes | X | X | ||
| ubl-1 | H06I04.4 | Ribosomal S31 fused to ubiquitin | U | Low | Diffuse | Low | Diffuse | No | X | X |
| unc-112 | C47E8.7 | PH domain mitogen-inducible gene-2 | Low | No | X | |||||
| unc-32 | ZK637.8 | Vacuolar ATPase subunit | V | Low | Yes | |||||
| vha-13 | Y49A3A.2 | Vacuolar ATPase subunit | V | Low | Yes | X | X | |||
| vha-19 | Y55H10A.1 | Vacuolar ATPase subunit | V | Low | Yes | |||||
| vha-2 | R10E11.2 | Vacuolar ATPase subunit | V | Low | No | X | X | |||
| vps-11 | R06F6.2 | Vacuolar protein sorting factor | V | Low | No | X | ||||
| zen-4 | M03D4.1 | Plus-end kinesin-like motor protein | C | Und. | Yes | |||||
| zyg-9 | F22B5.7 | Microtubule-association protein | C | Low | Diffuse | Yes | ||||
| AT-rich areaa | Low | No | X | |||||||
| B0361.10 | SNARE synaptobrevin/VAMP | G | Low | No | X | |||||
| C01G10.14 | Major sperm protein (MSP) domain | Low | No | X | ||||||
| C06A5.1 | Putative integrator complex subunit | S | Low in late embryo | Yes | X | X | ||||
| C34D1.2 | DM DNA-binding domain | Low | No | X | ||||||
| C35A5.4 | Major sperm protein (MSP) domain | Low | No | X | ||||||
| C41G7.3 | TSPO homolog. Putative cholesterol transporter | High in P cells | Large granules in early embryo | No | X | X | ||||
| C42D4.13 | Unknown | Low | No | X | ||||||
| F01G4.3 | DEAD/DEAH box helicase domain | Low | Diffuse | No | X | |||||
| F15E11.5a | Unknown | Low | No | X | ||||||
| F22B5.10 | TMCO1 homolog | C | Und. in some | No | X | X | ||||
| F29G6.1 | Serine protease inhibitor domain | Low | Diffuse | No | X | |||||
| F55C5.7 | Protein kinase, MIT, PX domains | Low | No | X | ||||||
| F59D6.7 | Calcium-binding pH regulator | Low | No | X | ||||||
| F59E10.3 | ζ-Subunit of coatomer (COPI) | G | Low | Diffuse | Low | No | X | |||
| F59E12.11a | Unknown | Diffuse | No | X | X | |||||
| K06H7.1 | Serine/threonine protein kinase | Low | Diffuse | Yes | ||||||
| K12H4.3 | rRNA maturation of 60S ribosome | Low | No | X | ||||||
| T03G6.3 | Phosphodiesterase | Low | No | X | ||||||
| T04A8.7 | Glucosidase acid β-orthologs | Low or und. | No | X | X | |||||
| T06E6.1 | Possible 60S ribosomal Nop7 factor | Low | No | X | ||||||
| T12D8.6 | Calmodulin containing | Low or und. | Yes | |||||||
| T19C3.6 | Unknown | Low | No | X | ||||||
| Y47D3A.29 | DNA polymerase primase | Low | Diffuse in P cells | Yes | ||||||
|
ZC477.4 |
Unknown |
Low |
No |
X |
||||||
a
Targeted gene originally misannotated in Ahringer RNAi Library. Correct sequenced target is shown.
b
Gene obtained more than once in the screen.
c
Gene class letter code (see Table 2).
d
Und., undetectable.