Fig. 3.

Imaging of laminin matrix in live motile and non-migrating keratinocytes. (A) Keratinocytes expressing YFP-tagged laminin-332 (green) were plated onto a glass coverslip and visualized by confocal microscopy 8 hours later. Phase-fluorescence overlays at the indicated time points are shown. This set of images was taken from Sehgal et al. (Sehgal et al., 2006), with permission. Scale bar: 50 μm. (B-D) A group of live, stationary keratinocytes expressing mCherry-tagged laminin-332 was viewed by confocal microscopy. The fluorescence and phase contrast images in B and C are overlaid in D. Scale bar: 20 μm.