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. 2009 Nov 24;122(24):4526–4534. doi: 10.1242/jcs.054536

Fig. 5.

Fig. 5.

Localization of Rab11 in dividing spermatocytes depends on bru function. (A-D) Localization of Rab11 in +/+ (wild type) and bruZ3358/Df(2L)pr2b (bru) spermatocytes. (A) Immunolocalization of endogenous Rab11 in telophase spermatocytes (left panels). The right panels show anti-Centrosomin (Cnn) staining (red) to label the cell poles and DAPI-stained DNA (blue). Arrows indicate the cell midzones. In the bru mutant spermatocyte, Rab11 does not form clear puncta at the cell poles or concentrate at the cell midzone. (B-D) Immunolocalization of Rab11-GFP in fixed wild-type and bru mutant spermatocytes stained with DAPI (DNA). (B) Prophase. (C) Anaphase. In the wild-type spermatocyte, an arrow indicates the parafusorial membranes that envelope the meiotic spindle. (D) Late telophase spermatocytes. Arrows indicate the cell midzones. Scale bars: 10 μm.