Skip to main content
. 2009 Nov 24;122(24):4558–4569. doi: 10.1242/jcs.050724

Fig. 1.

Fig. 1.

MMP-dependent HT1080 cell invasion in 3D collagen matrices precedes the appearance of MMP-independent invasive behavior. (A) nuc-GFP HT1080s were seeded in 3D collagen matrices with LPA and stimulated to invade for 48 hours in the presence or absence of 5 μM GM6001. Data are expressed as mean velocity of invading cells ± s.d. (error bars) for n=10 cells per condition over the indicated time intervals. (B) Assays set up as above were stained for tunnels using an anti-type-I-collagen antibody that only recognizes native collagen in ELISA assays, as shown in the right panel. (C-E) Assays were quantitated for invasion velocities (n=10 cells) using real-time analysis in the absence or presence of GM6001 at 5 μM (C) or 20 μM (D). Statistical significance *P<0.001; #, not statistically significant.. (E) Representative tracings of HT1080 motility are shown under the indicated conditions and time intervals. (F) Conditioned medium obtained from the indicated cultures and time points were analyzed using gelatin zymography.