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. 2009 Dec 2;106(51):21655–21659. doi: 10.1073/pnas.0912384106

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Fig. 3. — Coimmunoprecipitations indicate interactions of RISC factors with chromatin remodeling factors. (A) Lysates collected from Ago2-Flag (Ago-2) or the Flag nonspecific control (Flag) expressing cell lines were coimmunoprecipitated with an anti-Flag antibody. The Western blot was probed with antibodies against Topo 2α, EzH2, and Mta2. An antibody against HSP90 was used as loading control. (B) Lysates collected from Ago2-Flag (Ago-2) expressing cell lines were coimmunoprecipitated with an anti-CRM1 antibody with (+) or without (−) RNase added to the reaction. IgG, nonspecific antibodies of the same type and species were used as a nonspecific control. The blot was probed with specific antibodies against Topo 2α, EzH2, and Mta2. An antibody against α-tubulin (α-Tub) was used as loading control.