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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Vision Res. 2009 Feb 1;49(6):615–621. doi: 10.1016/j.visres.2009.01.012

Figure 5.

Figure 5

Quantitative analysis of Cre-mediated recombination at gp130 locus in Müller cells by detecting phosphorylated STAT3 (pSTAT3), an activated downstream target of gp130, in response to leukemia inhibitory factor (LIF). A–E: Single slice (0.5 μm) confocal microscopic images of pSTAT3 in inner nuclear layer of VMD2-cre−/gp130f/f mice (A–C) and VMD2-cre+/gp130f/f mice (D–F). GS: glutamate synthetase. Scale bar: 25 μm. G: Frequency of Cre-mediated recombination in Müller cells. Error bars: standard errors. ***: p<0.001 in t-test. Activation of STAT3 (pSTAT3) was detected by IHC 30 min after LIF injection. While LIF induced activation of STAT3 in all Müller cells in VMD2-cre−/gp130f/f mice (A, C) in response to LIF, STAT3 was not activated in 52.1% of Müller cells in VMD2-cre+/gp130f/f (arrowheads in D–F), suggesting that Cre-mediated recombination occurred in 52.1% of Müller cells at gp130 locus.