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. 2000 Aug 1;97(18):10282–10287. doi: 10.1073/pnas.160041997

Figure 1.

Figure 1

The effects of influenza virus on Na+ channel activity in tracheal epithelium. (a) Original recordings showing the response of the tracheal epithelium to 10 μmol/liter amiloride under (i) control conditions, (ii) after 1 h exposure of the apical membrane to UV-inactivated PR8 (106 pfu/ml before inactivation), or (iii) after 1 h apical exposure to active PR8 virus (106 pfu/ml). (b) Concentration–response curve for amiloride. Iammax is the amiloride-sensitive current determined by using a maximal concentration of amiloride, 100 μmol/liter. (c) The effects on amiloride-sensitive current of 1 h apical treatment with: (i) active PR8 influenza virus (106 pfu/ml); (ii) UV-inactivated PRB (106 pfu/ml); (iii) active WSN33 influenza virus (106 pfu/ml); (iv) replication-deficient adenovirus, MX17 (106 pfu/ml); and (v) allantoic fluid not infected with any virus. (d) Time course of the decline produced by 106 pfu/ml PR8 in the amiloride-sensitive current. (e) Viral concentration–response curve for inhibition of the amiloride-sensitive current after apical exposure to purified PR8 for 1 h. In a–e, Iam is the amiloride-sensitive current, and Iam0 is the amiloride-sensitive current before the addition of virus.