Table 2.
Quantification of Picolinyl ester derivatives of N. attenuata leaf extracts and comparison with two additional analytical methods.
| Treatments | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 min | 30 min | 60 min | ||||||||||
| wound | FAC | wound | FAC | |||||||||
| Substance | RT | [M+H]+ | Picol. esters | Method 2 or 3* | Picol. esters | Method 2 or 3* | Picol. esters | Method 2 or 3* | Picol. esters | Method 2 or 3* | Picol. esters | Method 2 or 3* |
| ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | ng gFW-1 (± SE) | |||
| JA-Ile | 6.86 | 415 | - |
1.1 (± 0.14) |
39 (± 4.0) |
46 (± 4.8) |
92 (± 6.0) |
81 (± 2.0) |
83 (± 9.0) |
97 (± 4.0) |
158 (± 16) |
163 (± 19) |
| SA | 8.77 | 230 |
193 (± 31) |
173 (± 23) |
203 (± 24) |
212 (± 19) |
182 (± 15) |
196 (± 11) |
216 (± 22) |
189 (± 17) |
116 (± 18) |
129 (± 22) |
| ABA | 9.01 | 356 |
257 (± 7) |
225 (± 8.0) |
192 (± 35) |
246 (± 9.0) |
274 (± 16) |
232 (± 14) |
264 (± 6.0) |
242 (± 10) |
277 (± 12) |
270 (± 14) |
| ICA | 9.08 | 253 | - | - | - | - | - | - | - | - | - | - |
| CA | 9.34 | 240 | - | - | - | - | - | - | - | - | - | - |
| JA | 9.43 | 302 |
137 (± 20) |
152 (± 23) |
937 (± 150) |
845 (± 95) |
1845 (± 125) |
1586 (± 110) |
1564 (± 180) |
1326 (± 42) |
3820 (± 350) |
3149 (± 223) |
| Traumatol | 9.85 | 306 | - | - | - | - | - | - | - | - | - | - |
| dnOPDA | 9.85 | 356 | - | - | - | - | - | - | - | - | - | - |
| IAA | 9.94 | 267 | - | - | - | - | - | - | - | - | - | - |
| Traumatin | 9.96 | 304 |
11.3 (± 5.6) |
14.4 (± 7.8) |
17.1 (± 4.8) |
15.2 (± 2.3) |
14.6 (± 3.4) |
12.4 (± 2.7) |
13.8 (± 1.9) |
15 (± 5.2) |
10.7 (± 3.6) |
12.2 (± 1.6) |
| Tr. acid** | 10.15 | 320 | - |
5 (± 0.96) |
19.6 (± 3.2) |
24.3 (± 6.7) |
23.8 (± 6.1) |
20.4 (± 4.8) |
17.1 (± 2.5) |
19.6 (± 2.3) |
20.9 (± 1.9) |
21.4 (± 4.2) |
| OPC4:0 | 10.46 | 330 | - | - | - | - |
12.6 (± 8.1) |
- |
10.3 (± 4.9) |
- |
18.5 (± 4.7) |
- |
| OPC6:0 | 10.67 | 358 | - | - | - | - |
5.3 (± 2.9) |
- |
8.6 (± 3.5) |
- |
11.2 (± 5.6) |
- |
| OPDA | 10.89 | 384 |
107 (± 60) |
91 (± 53) |
323 (± 47) |
386 (± 45) |
291 (± 11) |
322 (± 15) |
180 (± 45) |
184 (± 15) |
301 (± 31) |
313 (± 3.0) |
| GA3 | 11.27 | 438 | - | - | - | - | - | - | - | - | - | - |
| OPC8:0 | 11.41 | 386 | - | - | - | - | - | - | - | - | - | - |
| GA4 | 11.99 | 424 | - | - | - | - | - | - | - | - | - | - |
| 18:3 | 12.83 | 370 |
446 (± 38) |
398 (± 24) |
480 (± 53) |
497 (± 37) |
523 (± 30) |
497 (± 42) |
605 (± 20) |
596 (± 31) |
476 (± 33) |
424 (± 31) |
| 16:1 | 13.2 | 346 |
52 (± 9) |
26 (± 23) |
41 (± 3.0) |
22 (± 20) |
48 (± 12) |
51 (± 32) |
91 (± 26) |
147 (± 96) |
103 (± 13) |
121 (± 83) |
| 16:2 | 10.93 | 344 | - | - | - | - | - | - | - | - | - | - |
| 16:3 | 10.29 | 342 |
45.6 (± 12) |
- |
41.1 (± 2.0) |
28.2 (± 23.0) |
64.1 (± 19) |
40.5 (± 21) |
130.2 (± 10) |
80.9 (± 39) |
37.2 (± 3.0) |
- |
| 18:2 | 14.22 | 372 |
245 (± 16) |
190 (± 17) |
251 (± 8.0) |
189 (± 13) |
316 (± 45) |
398 (± 23) |
377 (± 43) |
415 (± 26) |
333 (± 209) |
367 (± 25) |
| 16:0 | 14.53 | 348 |
21100 (± 3000) |
22300 (± 4150) |
16600 (± 930) |
14100 (± 2300) |
18600 (± 2800) |
15400 (± 4700) |
23200 (± 3200) |
21600 (± 3000) |
14600 (± 900) |
15600 (± 3300) |
| 18:1 | 14.86 | 374 |
625 (± 47) |
502 (± 66) |
603 (± 22) |
572 (± 72) |
805 (± 59) |
716 (± 28) |
791 (± 84) |
778 (± 47) |
894 (± 36) |
813 (± 51) |
| 18:0 | 17.59 | 376 |
46800 (± 12900) |
44600 (± 7300) |
47900 (± 1200) |
33400 (± 9300) |
49000 (± 5100) |
36700 (± 4600) |
45700 (± 6200) |
41300 (± 3300) |
39400 (± 5300) |
33000 (± 2700) |
Quantification of compounds was performed in triplicate (n = 3)
-: below limit of detection under the conditions tested
* Method 2: Analysis of underivatized molecules by LC-MS/MS, this method was used to quantify compounds in the upper list section
* Method 3: Analysis of methyl esters of FFA (FAMES) by GC-MS
** Tr. acid: Traumatic acid