Table 3.

Smoking Responsive Oxidant-related Gene Expression in Alveolar Macrophages¹

Category	Probe set ID	Gene symbol	Gene title	Nonsmokers % present2	Healthy smokers % present	Smokers/non-smokers (fold-change)	p value
Glutathione metabolism	202923_s_at	GCLC	glutamate-cysteine ligase, catalytic subunit	100	100	1.67	0.007
Redox balance	No smoking responsive genes in this category in the alveolar macrophages
Catalase/SOD	216841_s_at	SOD2	superoxide dismutase 2	100	100	-2.06	0.006
Oxidant scavengers	No smoking responsive genes in this category in the alveolar macrophages
Pentose phosphate cycle	213093_at	PRKCA	protein kinase C, alpha	95	100	1.86	0.037
Xenobiotic metabolism	205939_at	CYP3A7	cytochrome P450, family 3, subfamily A, polypeptide 7	58	10	-3.69	0.030
Bilirubin related	209236_at	SLC23A2solute carrier family 23, member 2		89	100	1.50	0.038
Ascorbic acid related	No smoking responsive genes in this category in the alveolar macrophages
Production of free radicals	No smoking responsive genes in this category in the alveolar macrophages

¹ Smoking responsive genes defined as fold-change smokers compared to nonsmokers >1.5 and p value < 0.05 in genes expressed in >50% of either nonsmokers or smokers. Bold type represents differentially expressed genes in both small airway epithelium and alveolar macrophages (see Table 2).

² % present refers to Affymetrix Detection Call of Present as a % of total numbers of healthy nonsmokers (n = 19) and healthy smokers (n = 30).