Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Clin Cancer Res. 2009 Nov 24;15(23):7144–7152. doi: 10.1158/1078-0432.CCR-09-1483

Figure 2. mAb 1339 overcomes the effect of BMSC on both IL-6 dependent and independent MM cell growth.

Figure 2

(A) INA-6 and XG-1 cells were cultured with BMSC and in the presence of isotype Ab control as well as different doses of mAb 1339 for 24-72 hours. Cell proliferation was assessed by [3H]thymidine uptake assay and presented as % change from control. (B) MM1S, MM1R and U266 cells were cultured with BMSC or 5 ng/ml of rIL-6 with and without 1 μg/ml of mAb 1339, for 48 hours. DNA synthesis was assessed by [3H] thymidine uptake assay and presented as counts per minute (cpm). Data represent mean +/- SD, (N=3), (P≤ 0.05 for all samples). (C) Primary CD138+ MM cells were incubated in the absence or presence of BMSC with and without mAb 1339 for 24 hours. mAb 1339 is able to overcome the promoting effects of BMSC on MM cell growth.