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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Differentiation. 2009 Aug 19;78(5):253–259. doi: 10.1016/j.diff.2009.07.009

Figure 5. miR-133b overexpression downregulates IGF-IR posttranscriptionally.

Figure 5

(A) COS7 cells were transfected with luciferase reporter vectors carrying wildtype (pMIR-IGF1R-3’UTR) of mutant (pMIR-IGF1R-3’UTR-M) version of the potential miR-133b target sequence in the 3’UTR of IGF-IR gene. The cells were also transfected with miR-133b (black bar) or miRcontrol (white bar) and pRL-TK (internal control expressing Renilla luciferase activity). The translational regulatory activity of the wildtype or mutant IGF-I 3’UTR was presented as a ratio (relative expression) between Firefly and Renilla luciferase activities, based on 3 independent experiments. * indicates significant difference (P <0.05) compared to miR-control. (B) ADSC were transfected with pMIR-IGF1R-3’UTR and pRL-TK, treated with the indicated reagents at bottom, and then analyzed for Firefly and Renilla luciferase activities. The translational regulatory activity of the 3’UTR of IGF-IR gene was presented as a ratio (relative expression) between Firefly and Renilla luciferase activities, based on 3 independent experiments. * indicates significant difference (P <0.05) compared to untreated control.