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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: DNA Repair (Amst). 2009 Oct 31;8(12):1452–1461. doi: 10.1016/j.dnarep.2009.09.010

Figure 2.

Figure 2

Rad9 BRCT mutants initiate variable responses to uncapped telomeres. (A) rad9Δ strains were transformed with constructs harboring wild type RAD9-MYC or MYC-tagged rad9 mutant alleles, and analyzed for Rad53 activation in response to 300 Gy of ionizing irradiation. Samples were treated with nocodazole, irradiated and were harvested at 0, 15 and 30 min after irradiation. rad9Δ cdc13-1 strains were transformed with constructs harboring wild type RAD9-MYC or MYC-tagged rad9 mutant alleles, and analyzed for (B) Rad53 activation, H2A phosphorylation and (C) DNA content. (D and E) Microcolony analysis of the R1085E and K1088E mutants with the cdc13-1 allele. The data shown are the average of 3 replicates, mean ± SD. Bar = 10 μm