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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: DNA Repair (Amst). 2009 Oct 31;8(12):1452–1461. doi: 10.1016/j.dnarep.2009.09.010

Figure 3.

Figure 3

Rad9 binds to sub-telomeric chromatin after telomere uncapping. (A) A schematic representation of the sites probed within Chromosome VI for chromatin immunoprecipitation assays. (B) rad9Δ cdd13-1 strains expressing Myc-tagged RAD9 or rad9 mutant constructs were treated with α-factor at room temperature for one hour and then shifted to 37°C for 30 minutes. Next cells were released into pre-warmed YPD and incubated at 37°C. Samples were collected at 30 min, 1 h and 2 h time points and then used for ChIP analysis. Error bars represent the standard deviation between at least two replicate experiments. (C) With the same time course used in ChIP assays, Rad53 activation was detected after incubating cells at restrictive temperature. Arrows indicate high molecular weight species that correspond to phosphorylated Rad53. (D) DNA content analysis of cells from figure 3C after incubation at restrictive temperature.