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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 4;1797(1):29–37. doi: 10.1016/j.bbabio.2009.07.008

Fig. 1.

Fig. 1

A, Cell viability. Cells were incubated in glucose (grey) or galactose medium (black). Cell viability was then determined after 48 hours of growth in the indicated medium using the MTT assay. MTT absorbance value at time 0 was considered as the 100% value of viable cells. The number of viable cells was the average of three measurements. B, Oxygen consumption rates (nmol O/min/million cells) normalized to protein concentration. C&D, The rate of mitochondrial ATP synthesis was determined in the cells. ATP synthesis driven by complex I (glutamate plus malate) or complex II (succinate with rotenone as inhibitor for complex I) substrates with or without oligomycin was determined. The rates of mitochondrial ATP synthesis (nmol ATP/min/mg protein) represent the oligomycin sensitive reaction. The asterisk, * (P<0.05), indicates that the significant difference in cells containing cancer mitochondria, including cancer parental cells or their cybirds, when compared to 10A parental cells or its cybrids. Each measurement was performed in triplicate.