Table 1.
“Abreviated name” - Full Mutant designation | |
---|---|
Primer sequence (5′→3′) Mutant sequence underlined |
|
“2” - pΔE-C1hPARG59Δ2-4MTS | |
CTAGTCGACCCTCGGTGTGGGATCC CTATGGTACCTTCGTAAGTGACATGCAATCG |
|
“3” - pΔE-C1hPARG59Δ2-16MTS | |
CTAGTCGACTCTGCCAATCACACAGTAAC CTATGGTACCTTCGTAAGTGACATGCAATCG |
|
“4” - pΔE-C1hPARG59Δ2-24MTS | |
CTAGTCGACCGGGTAGATCTTTTGCG CTATGGTACCTTCGTAAGTGACATGCAATCG |
|
“5” - pΔE-C1hPARG59Δ15-26MTS | |
GATCTTTTGCGAGCAGGAGAAGTTCC CAAGAGAGGCAGCCGGATCCCA |
|
“6” - pΔE-C1hPARG59ΔR2A | |
CAGATCCGCTAGCATGGCAAGAATGCCTCGGTGTGG CCACACCGAGGCATTCTTGCCATGCTAGCGGATCTG |
|
“7” - pΔE-C1hPARG59ΔR3A | |
GATCCGCTAGCATGAGAGCAATGCCTCGGTGTGGGATC GATCCCACACCGAGGCATTGCTCTCATGCTAGCGGATC |
|
“8” - pΔE-C1hPARG59ΔR6A | |
CATGAGAAGAATGCCTGCGTGTGGGATCCGGCTGC GCAGCCGGATCCCACACGCAGGCATTCTTCTCATG |
|
“9” - pΔE-C1hPARG59ΔR10A | |
GCCTCGGTGTGGGATCGCGCTGCCTCTCTTGAGAC GTCTCAAGAGAGGCAGCGCGATCCCACACCGAGGC |
|
“10” - pΔE-C1hPARG59ΔR2A/R3A | |
GATCCGCTAGCATGGCAGCAATGCCTCGGTGTGGGATC GATCCCACACCGAGGCATTGCTGCCATGCTAGCGGATC |
|
“11” - pΔE-C1hPARG59ΔR2A/R3A/R6A/R10A | |
GCCTGCGTGTGGGATCGCGCTGCCTCTCTTGAGAC GTCTCAAGAGAGGCAGCGCGATCCCACACGCAGGC |
|
“12” - pΔE-C1hPARG59ΔL11D | |
CGGTGTGGGATCCGGGACCCTCTCTTGAGACCAT ATGGTCTCAAGAGAGGGTCCCGGATCCCACACCG |
|
“13” - pΔE-C1hPARG59ΔL11D/L13D | |
TGGGATCCGGGACCCTGACTTGAGACCATCTGCC GGCAGATGGTCTCAAGTCAGGGTCCCGGATCCCA |
|
“14” - pΔE-C1hPARG59ΔL11D/L13D/L14D | |
GATCCGGGACCCTGACGACAGACCATCTGCCAATC GATTGGCAGATGGTCTGTCGTCAGGGTCCCGGATC |
|
For deletion of EGFP from pEC1hPARG59 | |
AGCTAGCATGAGAAGAATGCCTCGGTGTG CTATGGTACCTTCGTAAGTGACATGCAATCG |
|
MTS-EGFP vector construction | |
AGCAGAGCTGGTTTAGTGAACCGTCAGATC GCAGCTAGCTTCAAGTTTTGGGGTCGTGTAAAT |