Table 1.

Plasmids created for analysis and primers used

“Abreviated name” - Full Mutant designation
	Primer sequence (5′→3′)  Mutant sequence underlined
“2” - pΔE-C1hPARG59Δ2-4MTS
	CTAGTCGACCCTCGGTGTGGGATCC CTATGGTACCTTCGTAAGTGACATGCAATCG
“3” - pΔE-C1hPARG59Δ2-16MTS
	CTAGTCGACTCTGCCAATCACACAGTAAC CTATGGTACCTTCGTAAGTGACATGCAATCG
“4” - pΔE-C1hPARG59Δ2-24MTS
	CTAGTCGACCGGGTAGATCTTTTGCG CTATGGTACCTTCGTAAGTGACATGCAATCG
“5” - pΔE-C1hPARG59Δ15-26MTS
	GATCTTTTGCGAGCAGGAGAAGTTCC CAAGAGAGGCAGCCGGATCCCA
“6” - pΔE-C1hPARG59ΔR2A
	CAGATCCGCTAGCATGGCAAGAATGCCTCGGTGTGG CCACACCGAGGCATTCTTGCCATGCTAGCGGATCTG
“7” - pΔE-C1hPARG59ΔR3A
	GATCCGCTAGCATGAGAGCAATGCCTCGGTGTGGGATC GATCCCACACCGAGGCATTGCTCTCATGCTAGCGGATC
“8” - pΔE-C1hPARG59ΔR6A
	CATGAGAAGAATGCCTGCGTGTGGGATCCGGCTGC GCAGCCGGATCCCACACGCAGGCATTCTTCTCATG
“9” - pΔE-C1hPARG59ΔR10A
	GCCTCGGTGTGGGATCGCGCTGCCTCTCTTGAGAC GTCTCAAGAGAGGCAGCGCGATCCCACACCGAGGC
“10” - pΔE-C1hPARG59ΔR2A/R3A
	GATCCGCTAGCATGGCAGCAATGCCTCGGTGTGGGATC GATCCCACACCGAGGCATTGCTGCCATGCTAGCGGATC
“11” - pΔE-C1hPARG59ΔR2A/R3A/R6A/R10A
	GCCTGCGTGTGGGATCGCGCTGCCTCTCTTGAGAC GTCTCAAGAGAGGCAGCGCGATCCCACACGCAGGC
“12” - pΔE-C1hPARG59ΔL11D
	CGGTGTGGGATCCGGGACCCTCTCTTGAGACCAT ATGGTCTCAAGAGAGGGTCCCGGATCCCACACCG
“13” - pΔE-C1hPARG59ΔL11D/L13D
	TGGGATCCGGGACCCTGACTTGAGACCATCTGCC GGCAGATGGTCTCAAGTCAGGGTCCCGGATCCCA
“14” - pΔE-C1hPARG59ΔL11D/L13D/L14D
	GATCCGGGACCCTGACGACAGACCATCTGCCAATC GATTGGCAGATGGTCTGTCGTCAGGGTCCCGGATC
For deletion of EGFP from pEC1hPARG59
	AGCTAGCATGAGAAGAATGCCTCGGTGTG CTATGGTACCTTCGTAAGTGACATGCAATCG
MTS-EGFP vector construction
	AGCAGAGCTGGTTTAGTGAACCGTCAGATC GCAGCTAGCTTCAAGTTTTGGGGTCGTGTAAAT