. Author manuscript; available in PMC: 2010 Dec 8.

Published in final edited form as: Biochemistry. 2009 Dec 8;48(48):11546–11558. doi: 10.1021/bi901731c

Table 3.

Kinetic constants for mutants.

Enzyme	k_cat (s⁻¹)	K_M (mM)	k_cat/K_M (M⁻¹s⁻¹)
Wild type	6.8 ± 1.2	0.62 ± 0.38	1.1 × 10⁴
H45Q	0.0013 ± 0.003	4.4 ± 2.7	0.29
Y90F	0.0061 ± 0.006	2.0 ± 0.48	3.1
R162N	-^a	-^a	0.65
Y164F	nd^b	nd	nd

Velocity independent of substrate concentration.

Not detectable.