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. 2000 Aug 15;97(18):10288–10293. doi: 10.1073/pnas.180295197

Figure 2.

Figure 2

Regulation of Cox2 mRNA in granulosa cells. (A) Granulosa cells were isolated and incubated for 0 h or 5 h in the presence or absence of 100 ng/ml GDF-9, and the RNA was isolated and analyzed by Northern blotting using a Cox2 cDNA probe or a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe as a quantitative control. (B and C) Time course of Cox2 mRNA synthesis in granulosa cells as measured by using semiquantitative RT-PCR. Granulosa cells were cultured in the presence or absence of recombinant mouse GDF-9 (100 ng/ml), and RT-PCR analyses were performed using Cox2 and hypoxanthine phosphoribosyltransferase (Hprt) (control) primer sets. Each point on the graph represents the mean ± SEM from triplicate samples. The asterisk denotes significance (P < 0.05) of control versus GDF-9-treated samples at that timepoint.