Figure 5.

GDF-9 stimulates EP2 receptor mRNA synthesis. Semiquantitative RT-PCR analysis was used to analyze the expression of EP2 and Hprt (control) in granulosa cells treated with (+) or without (−) GDF-9. (A) Representative samples from 6-, 8-, 18-, and 24-h timepoints are shown. (B) The data are summarized as mean ± SEM for triplicate samples except at the 6-h timepoint, which is mean ± range for duplicate samples for the GDF-9-treated group. Similar results were seen when the experiment was repeated on several independent occasions. Single asterisk denotes significance (P < 0.05) of control versus GDF-9-treated samples at that timepoint; double asterisk denotes significance of P < 0.10.