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. Author manuscript; available in PMC: 2010 Dec 18.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Oct 24;390(3):1044–1050. doi: 10.1016/j.bbrc.2009.10.111

Figure 3. Accumulation of lariat intron in TFIP11-depleted nuclear extract.

Figure 3

A, Human β-globin pre-mRNA was incubated with HeLa nuclear extracts. For reconstitution assay, 20 ng of recombinant TFIP11 was added in the splicing reaction. B, TFIP11 expression in nuclear extracts of HeLa cells transfected with negative silencer or TFIP11 siRNA was detected by immunoblot using rabbit-generated antibody against TFIP11 and β–actin included as control. C, Quantitation of the lariat intron fraction. Error bar represents the standard error of mean. Statistically significant comparison data indicated by *, p < 0.05.