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. Author manuscript; available in PMC: 2010 Dec 25.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Oct 28;390(4):1149–1153. doi: 10.1016/j.bbrc.2009.10.106

Fig. 3.

Fig. 3

PDE6H phosphorylation in chicken and murine retina.

Immunoblot of normalized dark- and light-adapted chicken and murine retinal extracts probed with rabbit anti-phosphoT20 {TTGDAPTGPT(pT)PR} antibody recognizing the phosphorylated T20 residue of PDE6H or rabbit anti-pT35 antibody recognizing the phosphorylated T35 residue of PDE6γ. Phosphorylated T20 was not detected in mouse retinal extract. Presence of PDE6γ in each sample was confirmed by stripping and re-staining the filter with rabbit anti-native PDE6γ antibody. D, dark-adapted overnight; L, light-adapted to 1.67×10-1 W/cm2 for sixteen hours.