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Demethylcurcumin treatment increased reduced glutathione levels and attenuated basal levels of reactive oxygen species (ROS) in HT4 neuronal cells. HT4 cells were seeded in six-well plates (0.1 × 106/well). Cells were treated with the indicated concentrations of demethylcurcumin (DC) for (A) 12 h or (B) 24 h. ROS levels were estimated as based on DCF fluorescence. Results are expressed as mean ± SD, p < 0.05; *compared with untreated control.
