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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Sep 15;1801(1):31–41. doi: 10.1016/j.bbalip.2009.09.002

Figure 4.

Figure 4

Inhibition of the carboxylesterase activity of recombinant human CES1 by AA. The data were best fit to an equation that describes noncompetitive enzyme inhibition (A) [27]: i = [I]/([I] + Ki). For a noncompetitive inhibitor it is assumed that the E S I complex is catalytically inactive (i.e., β=0), and the affinity of substrate for enzyme is unchanged by the inhibitor (i.e., α=1). The combined data from 3–4 independent experiments are shown [error bars represent the intra-assay variation (SD) for each independent experiment, which were performed in triplicate]. Analysis of data using a Dixon plot (shown in B) followed by a replot of the slopes of the Dixon plot versus 1/[S] (shown in C) confirmed a noncompetitive mode of inhibition [28].