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Each of the thymines within the bcl-2 Py39 sequence was substituted with a fluorescent tricyclic naphthodeoxyuridine thymine analog. Fluorescence was plotted for each substituted thymine as relative fluorescence units over a pH gradient (A). Panel B displays the proposed folding pattern of the bcl-2 i-motif with identification of the substituted thymines. The yellow, green, red, and blue circles represent the nucleobases cytosine, adenine, guanine, and thymine, respectively.
