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. Author manuscript; available in PMC: 2010 Nov 20.
Published in final edited form as: Immunity. 2009 Oct 29;31(5):787–798. doi: 10.1016/j.immuni.2009.09.014

Figure 1. IL-17-deficient mice are more susceptible to B16/F10 melanoma development in the lung.

Figure 1

IL-17−/− (KO) and wild-type (WT) mice were challenged i.v. with B16/F10 melanoma and lungs were analyzed on days 14 and day 16. A. Photographs of the lungs. Graphs on the right show total number of tumor colonies present in the lung lobes (n=4, avg. +/− s.d). B–E. At day 16, lung leukocyte and lung cell fractions were isolated and processed for FACS analysis or RNA extraction. B. Total leukocyte cell numbers from lungs. The numbers were calculated from the percentages of total live cells that were gated on CD45+ cells (n=4, avg. +/− s.d.). C. Expression of CD44 on CD45+CD4+ T cells from the leukocyte fraction. D. Myeloid populations (n=4, avg. +/− s.d). E. Chemokine and chemokine receptor gene expression analyses from lung fractions, which were free of leukocytes. Shown are the averages of 4 mice after duplicate analysis per sample (n=4, avg. +/− s.d). Results shown are from a representative experiment of three, each using 4–5 mice per group.