Figure 6. Th17 T cell treatment promotes CD8⁺ effector T cell differentiation.

A–B. Purified CD8⁺ T cells from OT-I mice were labeled with CFDA-SE and transferred into C57BL/6 mice bearing 5-day established pulmonary B16 nodules (OT-I). On the day of the transfer, one group of mice also received OT-II Th17 (OT-I⁺ Th17). On day 3 after transfer, LLN, lungs and spleens from these mice were analyzed for T cell proliferation, IFN-γ and IL-2 production. A. Histograms show cell division. Numbers in the lower panel indicates numbers of division. B. Cytokine production. Numbers inside the plots represent the percentages of dividing OT-I cells that also produced the cytokine. Bar graphs indicate the percentages of cells that produced cytokines per cell division. C–D. CD8⁺ T cells from OT-I mice were labeled with CFDA-SE and transferred into C57BL/6 mice or IL17−/− (KO) bearing 5-day established pulmonary B16-OVA. LLN and inguinal lymph node (ILN) cells from these mice were analyzed for proliferation and cytokine production on day 4 after transfer. C. Total numbers of CD8⁺CFSE⁺ T cells recovered from ILN and LLN. D. Cytokine profiles of CD8⁺ CFSE⁺ T cells from LLN. E. Total number of CD8⁺CFSE^low IFN-γ⁺ T cells recovered from LLN.