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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Acta Biomater. 2009 Jul 14;6(1):29–38. doi: 10.1016/j.actbio.2009.07.009

Figure 7.

Figure 7

Endothelial migration into scaffolds containing HepG2 cells. Modular scaffolds were formed from all three types of microspheres in the presence of HepG2 cells stained with Vybrant DiO (green). After crosslinking with media proteins and hydrolysis of porogenic microspheres for 48 h, endothelial cells (red) were seeded onto all sides of the scaffolds. The scaffolds contained: (i) S1P-loaded PEG8-VS/BSA microspheres (blue) without RGD peptide, (ii) PEG8-VS/BSA microspheres (blue) loaded with S1P, with RGD, (iii) PEG8-VS/BSA microspheres (blue) not loaded with S1P, with RGD. Cells were imaged by confocal microscopy with a 10X objective. Endothelial cells migrated to the maximum observable distance (300 μm) within two days with or without S1P delivery. Scaffolds were imaged by confocal microscopy with a 10X objective.