FIG. 3.

Transgenic mice with doxycycline-inducible expression of glutathione peroxidase 4 (Gpx4). Tetracycline response element (TRE)/Gpx4 mice were generated as described in Methods and crossed with opsin/rtTA transgenic mice to generate Tet/opsin/Gpx4 double transgenic mice. Adult Tet/opsin/Gpx4 mice or littermates lacking one of the transgenes were given drinking water containing (+) or lacking (-) 2 mg/ml doxycycline. After 2 weeks, mice were euthanized, and retinal homogenates were assayed for protein concentration; samples containing 50 μg were run in immunoblots for Gpx4. The blots were stripped and reprobed for actin. There was an increase in Gpx4 in the retinas of Tet/opsin/Gpx4 mice treated with doxycycline.