Figure 2.

Workflow in a quantitative lipid analysis determination. Fragmentation of member species are conducted to determine the molecular species. Varying degrees of quantitation (e.g., absolute or relative) can be performed on data coming from either full or headgroup-specific scans in systems with or without an LC column. A series of data processing steps including but not limited to baseline subtractions, deisotoping, and peak matching to lipids positively identified by MS/MS are standard elements in a typical lipidomics system. The sophisticated use of higher order statistical analysis, often multivariate by nature, is growing in applications and importance.