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. 1998 May 26;95(11):6560–6565. doi: 10.1073/pnas.95.11.6560

Figure 4.

Figure 4

Circularization of (−)PSTVd RNA in planta. Duplicate sets of RPAs using greater-than-full length probes specific for (+)PSTVd (Left) and (−)PSTVd RNA (Right) are shown. Lanes: 1–4, purified in vitro PSTVd RNA transcripts; 5–8, total RNA isolated from transgenic plants expressing either (+)PSTVd (lane 5) or (−)PSTVd RNA (lanes 6–8). All plants were PSTVd-infected, and samples 5–8 each contained 30 pg of (+)PSTVd or(−)PSTVd RNA. Positions of RNA:RNA duplexes containing circular (C) and linear (L) target molecules are marked (arrows). Strands shown in black and gray correspond to (+) and (−) PSTVd sequences, respectively, and the asterisks indicate the strand used as assay probe. Note that circularized (−)PSTVd RNA is detectable only in those plants that express the corresponding linear molecule. Exposure time for the (−)PSTVd RNA analysis was 10-fold longer than that for the (+)PSTVd analysis.