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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Clin Cancer Res. 2009 Nov 17;15(23):7238–7245. doi: 10.1158/1078-0432.CCR-09-1964

Figure 4.

Figure 4

Protection against radiation-induced DNA DSBs. (A) The effect of tetracycline on radiation-induced DNA DSBs was assessed by γ-H2AX immunofluorescence foci formation at 15 min after irradiation at 2 Gy in human W-T LCLs. Tetracycline at 20 µM reduced radiation-induced foci by 33%. (B) Tetracycline (10 µM) or ciprofloxacin (5 µM) did not reduce radiation-induced ROS in Til1 cells. Tetracyclines and fluoroquinolone antibiotics stimulated Tip60 HAT activity in Hela cells to the level similar to the radiomimetic agent bleomycin (C) and in two human W-T LCLs (CHOC6 and NAT9) to a lesser degree (D). 5 µM bleomycin, 25 µM tetracycline, 10 µM ciprofloxacin, 10 µM levofloxacin, and 10 µM chlorotetracycline were utilized for the HAT assay.